Photomicroscopy and ImageJ

Photomicroscopy and ImageJ

Postby Roy Miller » Tue Apr 27, 2010 11:21 pm

Does anyone out there use ImageJ software and familiar with the calibration method to set up the Micrometer Scale onto the photos. If so could they enlighten me? Cannot seem to find the necessary in the User Guide.

Thanks
[color=#008000][b]Roy[/b][/color]
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Re: Photomicroscopy and ImageJ

Postby Leif Goodwin » Wed Apr 28, 2010 7:46 am

I cannot answer your question, but if the aim is to record images from a microscope, with calibration of scale, you should find that the Micam package is better than the software supplied with many scopes, and it supports calibration, and measurement. It is also shareware, and inexpensive.
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Re: Photomicroscopy and ImageJ

Postby Roy Miller » Thu Apr 29, 2010 9:42 pm

Thanks Leif.

FYI - ImageJ is Open Source code and is free at http://rsbweb.nih.gov/ij/

Brunel informed me that they believe that the software is used by several UK universities, but that the programme had a very steep learning curve, which I can personally vouch for. It has become blatantly obvious that I must live in Photomicrosopy's Dark Ages, as about 80% of ImageJ's content goes way over my head, but I have at least now managed to insert some calibrated scales onto photo's. If however, ImageJ overwhelms me, I shall fall back on your suggestion of the Micam package for Plan B.
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