Permanent slides

Permanent slides

Postby davidharries » Thu May 13, 2010 4:39 pm

Can anyone offer advice on a suitable method for preparing permanent slides, for example spore and squash mount preparations?

There seems to be plenty of web information available on techniques and stains for temporary mounts - but little on permanent mounts except for subjects such as insects, diatoms, plant material sections etc.

Currently I prepare a slide with 50/50 water/glycerol, sometimes with a drop of congo red, then seal around the coverslip with clear nail varnish. Seems to work well - but I doubt that these will last more than a few weeks or so.

David H.
http://www.pembsfungi.org.uk
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Re: Permanent slides

Postby Mal Greaves » Thu May 13, 2010 5:49 pm

David
I think you are doing all the right things and I am sure done well they will last far longer than you suggest. I also think there is much less need for permanent slides in this digital age. A slide may contain just one or two distinctive elements such as Cheilocystidia/Pleurocystidia or stellate hairs and to relocate them can be a nightmare, but a photo of the culprit is far easier to locate and compare with a current find.

Mal
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